β-lactamase Gene Sequencing to Determine Antibiotic Resistance of Elizabethkingia Anophelis in the Gene AG1-BL 1516

Abstract

Antibiotics are used to treat infections caused by bacteria in the body. However, many species are antibiotic resistant like Elizabethkingia anophelis. In our research, we wanted to determine which antibiotics it was resistant to. We needed to use PCR amplification by designing the primers so we could order them. We separated them by using a DNA agarose gel electrophoresis to separate by size. Then we purified the DNA using organic extractions. We then took a look at our ligation reactions: they seemed to be successful. Lastly, we use heat shock transformation to solidify our results, and plated them on an agar plate containing kanamycin.