The Effect of Overexpression of SKIP16 on Arabidopsis thaliana Reproductive Development

Caroline Sanders, Yixing Yang, Ming Yang

Abstract


In order to gain a better understanding of the function of SKIP16, a gene encoding the F-box protein of the SKP1-CULLIN-F-BOX PROTEIN (SCF) complex, on the reproductive development of Arabidopsis thaliana, we conducted an experiment with transgenic plants containing the gene ASK1:SKIP16. We did this to see the effect of overexpression of SKIP16 on the plants to further begin to understand the role of SKIP16 on Arabidopsis development since the ASK1 promoter ubiquitously drives high expression levels of SKIP16. While it is known that SCF complexes impact growth and development in plants, the function of SKIP16 is unknown. We observed that at the T1 generation, two of the ASK1:SKIP16 plants had only one terminal flower on each inflorescence stem while many other plants produced either infertile siliques or siliques with reduced fertility. These observations indicate that SKIP16 plays an important role in Arabidopsis reproductive development. Further research on the function of SKIP16 may yield crucial information about how SCF F-box protein regulates plant development in Arabidopsis and other plants.

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