Pan Genomes of Pathogenic Bacteria

Austin Temple, Haley Riley, Akhilesh Ramachandran, Wouter Hoff

Abstract


Antibiotics play a big role in today’s society, but with the ever growing antibiotic resistance of bacteria, the effectiveness of this type of treatment is diminishing. Researchers everywhere have been trying to understand the specific genes associated with antibiotic resistance in the hope of finding a better way to combat this threat. Our team decided to join in the search--exploring the resistance and genes of Pseudomonas and Enterococcus bacteria. Our hypothesis is that there will be a relationship between the resistance of the bacteria against certain antibiotics and the presence of a set or one particular gene. We plan on isolating bacterial samples, extracting their DNA, and testing them against a range of antibiotics. The data we gain from this exploration could greatly aid in the understanding of resistance in pathogenic bacteria.

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References


Harbottle, H., S. Thakur, S. Zhao, and D. G. White. 2006. Genetics of Antimicrobial Resistance. Animal Biotechnology 17: 111-124.

Healthcare-associated infections. 2014, May 7. Centers for Disease Control and Prevention. https://www.cdc.gov/hai/

Hrabak, J., E. Chudackova, and R. Walkova. 2013. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clinical Microbiology Reviews 26: 103-114.

Huycke, M., D. Sahm, and M. Gilmore. 1998. Multiple-drug resistant enterococci: the nature of the problem and an agenda for the future. Emerging Infectious Diseases 4: 239-249.

Joshi, R. S., M. D. Jamdhade, M. S. Sonawane, and A. P. Giri. 2013. Resistome analysis of mycobacterium tuberculosis: identification of aminoglycoside 2’-N-acetyltransferase (AAC) as co-target for drug desigining. Bioinformation 9: 174-181.

Krásný, L., R. Hynek, and I. Hochel. 2013. Identification of bacteria using mass spectrometry techniques. International Journal of Mass Spectrometry 353: 67-79.

Lister, P. D., D. J. Wolter, and N. D. Hanson. 2009. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clinical Microbiology Reviews 22: 582-610.

Mirsadraee, M. 2014. Anthracosis of the lungs: etiology, clinical manifestations and diagnosis: a review. Tanaffos 13: 1-13.

Rouli, L., V. Merhej, P.-E. Fournier, and D. Raoult. 2015. The bacterial pangenome as a new tool for analyzing pathogenic bacteria. New Microbes and New Infections 7: 72-85.

Salipante, S., D. Roach, J. Kitzman, M. Snyder, B. Stackhouse, S. Butler-Wu, C. Lee, B. Cookson, and J. Shendure. 2015. Large-scale genomic sequencing of extraintestinal pathogenic Escherichia coli strains. Genome Research 25: 119-128.

Stuart-Harris, C. H., and D. M. Harris. 1982. The control of antibiotic-resistant bacteria. Academic Press, London.

Antibiotic resistance threats in the United States, 2013. 2013. Centres for Disease Control and Prevention, U.S. Department of Health and Human ServicesAtlanta, GA.


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