DNA Binding Domain of INI-1 1-141 in Atypical Teratoid Rhabdoid Tumors

Rachel Terry, Jessica Matts, Donald Ruhl

Abstract


Atypical teratoid rhabdoid tumors (AT/RT) are a devastating form of pediatric brain cancer. Due to the lack of current treatment for this cancer, the percent survival rate amongst children who are diagnosed with this cancer is only 10%. The expression of AT/RT cancer has been linked to the absence of a protein within the SWI/SNF complex called INI-1. Our lab has shown that INI-1 binds to DNA in the N-terminal half within amino acids 1-186. Because DNA binding domains typically do not require 186 amino acids, we hypothesized that there is a smaller binding domain within the 1-186 binding region. This paper shows the expression and purification of several N-terminal truncations of INI-1.

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References


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