Increasing cellular respiration of fermented S. Cerevisiae using fuel with hydrolysis enzymes

Ashley Jantz, Jessica Ferman, Sarah Alexander, Benjamin Abram, Shannon Reeves

Abstract


The rate of cellular respiration for fermenting yeast can be manipulated by changing the concentration and chemical constitution of the carbohydrate source. While several studies have proposed the perfect ratio to use in fermentation for various carbohydrate sources, an analysis of hydrolysis enzymes versus no hydrolysis enzymes has yet to be investigated. Using honey and high fructose corn syrup in a yeast solution, the rate of fermentation was measured in ppm and analyzed using a One-Way ANOVA test.The findings suggest that fuel sources with hydrolysis enzymes, such as honey, do not have a significant effect on the fermentation of yeast because of the P-value (0.9940) determined through the One-Way ANOVA test did not suggest any correlation. While the hydrolysis enzymes, like a-amylase and invertase, found in honey should theoretically improve fermentation because they speed up the breakdown of starches into smaller monosaccharides, farther testing will be needed to determine whether this increases ethanol production.


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