Protein Activity Regulation Based on Proximity Hybridization

Abstract

Precise regulation of protein activity is of great significance for the study on the molecular mechanisms of biological processes and drug development. Through years of efforts, researchers have found numerous strategies to inhibit and restore protein activity, with an encouraging improvement to be achieved recently. A novel method for robust protein inhibition and restoration of protein activity, chosen thrombin as a model protein, which utilizes the formation of a closed-loop structure by proximity-dependent surface hybridization and NIR photons, has emerged and received great attention. The enhanced capabilities of this strategy ensure its promise for wide application in various real samples.