Filling in a gap for the DNA of Arhodomonas sp. Strain Seminole

Abstract

Arhodomonas is a bacterium that was found in crude oil contaminated soil. The problem was that when first sequenced, only segment contigs were found. Our group then was given two contigs and we hypothesized that the two pieces will fit together with a gap in between them and the gap could be sequenced by finding a related gene for the contigs. To determine if the contigs go together we used a fused contig sequence in a blast program compared to our two segments and our results were positive for a gap. Then we ran a blast program to determine what the protein was to fill the gap, which we discovered to be 1-aminocyclopropane-1-carboxylate deaminase.