Finding the DNA Gap in Arhodomonas Seminole Using DNA Sequencing

Abstract

Arhodomonas Seminole is an aerobic, halophilic bacterium enriched from salty, crude-oil-impacted soil in Seminole Co, OK. This is a newly found and researched bacteria. Samples of this bacteria were obtained through the instructor. This samples had been pre-ruptured, pre-purified, and pre-concentrated by the instructor so they were ready to be analyzed. These samples were then sequenced using Blastx at the NCBI website. After sequencing was completed, reeds were produced. By overlapping and correctly ordering these reeds, two contigs were formed and ready to be put together. Predicting adjacent contigs based on what is known in related species and other strategies were used. Ultimately, this was achieved by the PCR process. This process was focused solely on one small portion of the DNA. Designed PCR primers were then created and used to complete the PCR process. After PCR the copied DNA was viewed and examined by gel electrophoresis. After this process the final product was finally gathered, which is what will be presented to you here.