Discovering the Genomic Sequence of Arhodomonassp. Seminole by Polymerase Chain Reaction and Gel Electrophoresis

Abstract

Our goal was to discover a portion of the genomic sequence of Arhodomonas sp Seminole, which is important due to its ability to break down dangerous hydrocarbons in very salty soils. We did this by PCR to replicate the desired sequence and gel electrophoresis to separate it. Our results were unsuccessful in determining the results we desired as the PCR failed to produce a result, likely, due to a variety of reasons. According to our BlastX analyses, the sequence in question would likely have coded for the protein 3-ketoacyl-CoA thiolase, which is necessary in the breakdown of glucose during respiration. Despite the current failure, we believe that the current gap is correctly aligned and that rerunning the PCR with different primers then a product should be made.