Identifying a gap using DNA Sequencing in an Arhodomonas Related Protein

Abstract

Arhodomonas sp. Seminole is a bacterium found in soil around oil rigs and other high-salt conditions. Arhodomonas’ ability to adapt to such conditions is intriguing and should be better understood. We are attempting to solve the bacterium's genome for a better grasp on how Arhodomonas survives in such extreme conditions. We focused on one small region of DNA and replicated it using a Polymerase Chain Reaction (PCR). After completing the PCR we received a reverse sequence that aligned with the contig upstream of our gap. From this information we were able identify that the contig and sequence match up. This is a step in the right direction. If we were to go back and redesign the primers we would most certainly sequence the gap with both a forward and reverse DNA sequence. Allowing us to close yet another gap in the genome of Arhodomonas sp. Seminole.