The Sequencing of Arhodomomas Species Seminole Using PCR in Order to Better Analyze the Predicted Sequence Gap

Abstract

The objective of this project was to fill in a gap of a genomic sequence of Arhodomonas sp. Seminole. This is a bacterium that lives in salty, crude oil impacted soil in Seminole Co, Oklahoma. A program called Blastx was used to design a PCR primer which would allow the desired sequence to be made. Polymerase Chain Reaction or PCR, which is a biochemical technique used to amplify a specific piece of DNA by generating millions of copies of a particular DNA sequence done by splitting open cells to purify DNA and then concentrating the precipitation. After this gel electrophoresis was used to analyze the results. Our results showed that we did not choose the specific region in the DNA sequence that was needed.