Genome Sequencing on Arhodomonas sp. Seminole to Determine Genetic Identity

Abstract

Arhodomonas sp. Seminole is a member of the genus Arhodomonas in the class Gammaproteobacteria. With each research group receiving a region to sequence, the project’s goal was to sequence the genome of the bacterium in order to better understand the species role in the environment and potential benefit in the process of oil field recovery. The first step was to run the contigs through BlastX to determine if there was an alignment. Next, we had to use Primer Quest to determine the forward and reverse primers for the PCR process. After obtaining the primers, we began the PCR process by extracting the DNA from a sample of Sp. Seminole. After copying the DNA, we viewed it by using gel electrophoresis. The PCR happened to have failed for our group. Running a hypothetical fused contig through BlastX yielded outstanding matches with multiple proteins, but the best result was flotillin, a related bacteria Acinetobacter Tandoii with a 77% match to Arhodomonas sp. Seminole.