Examination of a DNA Gap in Arhodomonas sp. Seminole Using PCR and DNA Sequencing

Abstract

The objective of the experiment was to attempt to fill in an unknown gap in the DNA of Arhodomonas sp. Seminole. We examined two known DNA contigs from the bacterium for potential alignment with a fused sequence of the two. Potential alignment was observed so we compared the fused sequence with a related protein sequence to design primers for PCR. Possible  primers were designed to attempt PCR sequencing of the gap area. This was followed by agarose gel electrophoresis which was unsuccessful.