The Search For the Hidden Genome Sequence: Seminole Style

Abstract

 Arhodomonas sp. Seminole is a bacterium found thriving in crude-oil-impacted soil in Seminole County, Oklahoma. It requires salt to live and grows on a wide variety of carbon sources. After a soil sample was taken and analyzed scientists working on this project discovered that the genome sequence was incomplete resulting in 750 contigs. In an attempt to sequence these gaps we used PCR to replicate the space between two contigs. Upon receiving a positive result we used that information to align the sequences and discovered 1-Aminocyclopropane-1-carboxylic acid (ACC).