FINDING (N)EMO: Sequencing the track of n’s between fused contigs and the DNA sequence

Abstract

We were presented with the bacterium Arhodomonas sp. Seminole, the full DNA sequence of which is currently unknown. Our job was to track the DNA of a missing contig by utilizing database resources, such as BLASTX, and the technique of PCR amplification. Our group had a positive forward PCR product, which led us to the conclusion that two fragments of the original sequence and forward primer had aligned.