Genomic DNA Sequencing of Arhodomonas sp. Seminole

Abstract

The objective of the experiment was to determine the missing amino acids in the DNA sequence for Arhodomonas. We were looking to fill in the gap between two contigs by using forward and reverse primers in order to fill the entire genome sequence. DNA was extracted from the bacteria; primers were designed for the PCR procedure. PCR produced millions of copies of the DNA for examination. Once the PCR was complete, the missing pieces of the DNA sequence were determined. The PCR product was examined via Agarose Gel Electrophoresis by the bands produced in the gel. Once the missing base pairs were determined, the bacteria was characterized by gene according to its phenotype. By finding the base pairs in all of the gaps in the sequence, the genome will be complete. A complete genome yields the ability for the degradation of hydrocarbons in the oil-filled waste to be understood.