Sequencing Arhodomonas sp. seminole: An Oil Well’s Maid

Abstract

In this project, our goal was to sequence the gap between two contigs of Arhodoonas sp. Seiminolen DNA, an bacterium found in the salty, oil saturated environments of well sites and use PCR to determine if the hypothesized protein fit the gap. After sequencing the DNA, we noted the gap between contigs was small and the protein extended over the hypothesized gap. We designed primes for the sequence. PCR was our next step, in which we put our primers to the test. The PCR was run several times, but came back negative under normal biological conditions. Another test suggested a better protein for the gap would be a formate transporter.