β-lactamase Gene Sequencing to Determine Antibiotic Resistance of Elizabethkingia Anophelis

Abstract

Antibiotics are used to treat infections caused by bacteria in the body. However many species are antibiotic resistant like Elizabethkingiaanophelis. In our research we wanted to determine which antibiotics it was resistant to. We sequenced the genomic DNA; then we purified the DNA using organic extractions. We also needed to use PCR amplification by designing the primers so we could order them. We separated them by using a DNA agarose gel electrophoresis to separate by size. We then took a look at our ligation reactions: They seemed to be successful. Lastly we use heat shock transformation, to determine our results.