The Presence of Protection from Reactive Oxygen Species Proteins in Elizabethkingia anophelisin antibiotics Cefotax and Imipenem

Abstract

Not much is known about the bacteria Elizabethkingia anophelis, and this project focuses on finding a pathway of genes and seeing if these genes are transcribed within the cell for it to use. The project involves looking at mRNA sequences for the genes that are involved in the Reactive Oxygen Species subcategory of the Oxidative Stress category of E. anophelis. The first phase of the research looked at finding the DNA sequences for each gene and finding if the sequences are found in any of three environments: the control, Cefotax, and Imipenem (antibiotics). Then, the second phase was looking at the map of the genome to see where the genes were located and what the surrounding genes were. The last phase was finding exactly how many copies of mRNA were found in the E. anophelis cells when it was submerged into each of the three different environments that included the two antibiotics.