The Effects of Antibiotics on Biotin Development

Abstract

First we decided on a function of the of the bacteria within the RAST database. We decided on the process in which the cell produces biotin, a vitamin essential to the bacteria’s life involved heavily in the production of fatty acids and the metabolism of various fats and amino acids. This is called “biotin biosynthesis.” Next each of us found two genes within the RAST database pertaining to this particular function. To find out more information about the two genes we each selected, we looked at both surrounding genes as well as the DNA sequences. We used the BLAST site to make sure the sequences we found on the RAST database matched the actually, full DNA sequence of E. Anophelis. Lastly we searched an excel document provided to us by the professor containing the entire transcriptomeof E. Anophelis. Once there we were tasked with finding each each and noting down the total number of transcriptions from three separate experimental groups. One grown with the addition of Cefotaxime, one with Imipenemand a control.