Elizabethkingia and the PCR Product

Abstract

The purpose of this project was to figure out which bacteria are resistant to antibiotics, which are a type of medicine which are used to treat bacterial infections. For every single human cell, there is a bacterial cell present. The mechanisms of antibiotic resistance are to pump the antibiotic out of the cell, destroy the antibiotic, modify the antibiotic, and modify the antibiotic and to “dodge” the antibiotic. The specific species we were working with was the ElizabethkingiaAnophelis, which is found in the gut of mosquitos and causes human disease. There were several steps in the process of this project and they were; Sequence the DNA, Extracting DNA from samples of interest, Sequence the Genomic DNA and determine what genes exist, PCR amplification, PCR fragment for cloning, put into E. Coli, Design primers, ligation reactions, heat shock transformation, and finally screen for Beta-Lactamase activity.