Determining that the E.ano_Ag1_bla267 gene in Elizabethkingia anophelis bacteria codes for Beta-lactamase

Abstract

The focus of this experiment was to identify beta-lactamase-coding genes in Elizabethkingia anophelis bacteria. We were presented with the gene E.ano_Ag1_bla267 that had indicators that it might be a beta-lactamase gene. To determine if this gene is a true beta-lactamase gene, we conducted PCR, inserted the amplified E. anophelis DNA into vectors, inserted the resulting vectors into E. coli, and identified true recombinant clones and tested them for beta-lactamase activity. We found that our gene is a strong positive. In conclusion, we determined that the E.ano_Ag1_bla267 is a true beta-lactamase-coding gene.