Increased ethanol production through S. cerevisiae fermentation in tap water compared to deionized water

Hunter Pickens, Rachel Hamrock, Tyler Perez, Ben Miskin, Sihan Yu


Baking and brewing companies are interested in maximizing the production of ethanol through S. cerevisiae, baker’s yeast, fermentation. We are interested in comparing how tap water and deionized water affect yeast fermentation and the amount of ethanol produced. To compare the water types, we ran trials measuring the amount of ethanol produced through yeast fermentation with tap water compared to the same trials with deionized water. Our study showed that under the same conditions, yeast fermented in tap water had a larger average ethanol production than yeast fermented in deionized water. Our results suggest that the additional contaminants in tap water can maximize ethanol production in yeast fermentation.

Full Text:



Environmental Working Group. (n.d.). More Ethanol Means More Toxic Water Pollution.

French, D. P. 2019. Investigating Biology: A Laboratory Resource Manual. 2019 Edition.

Fountainhead Press: South Lake.

Maltman, A. 2019. The Geology of Wine, Spirits and Beer. Reference Module in Earth Systems

and Environmental Sciences.

Rajagopalan, N., V. Singh, B. Panno, and M. Wilcoxon. 2010. Use of cooling tower blow down

in ethanol fermentation. Water Science and Technology 62:2263–2269.

Ruttarattanamongkol, K., M. E. Wagner, and S. S. Rizvi. 2011. Properties of yeast free bread

produced by supercritical fluid extrusion (SCFX) and vacuum baking. Innovative Food Science & Emerging Technologies 12:542–550.

Saeed, M. A., Q. Wang, Y. Jin, S. Yue, and H. Ma. 2019. Assessment of Bioethanol Fermentation

Performance Using Different Recycled Waters of an Integrated System Based on Food Waste. BioResources 14(2):3717–3730.

Udomsaksakul, N., K. Kodama, S. Tanasupawat, and A. Savarajara. 2018. Diversity of ethanol

fermenting yeasts in coconut inflorescence sap and their application potential.

ScienceAsia 44:371.

Zagala, R. 2018, July 16. Understanding the Benefits and Risks of Deionized (DI) Water.


  • There are currently no refbacks.